郭光沁（guang-凯发k8娱乐

research interests

1、 细胞分裂素和生长素相互作用 auxin/cytokinin interactions   

    生长素和细胞分裂素是调控植物发育的两类重要激素，其比例和相互作用决定着细胞生长和分化的命运。我们用分子遗传学等相关技术研究细胞分裂素如何影响生长素生物合成及极性运输进而调控植物的生长发育。

    auxin and cytokinin are the two classic plant hormones playing pivotal roles in plant development. it is a famous theme in plant science that the development of cells into roots or shoots is often determined by the ratio of auxin/cytokinin. we use molecular genetics to studies how cytokinin regulates auxin biosynthesis and its polar transport to influence plant development.

2. 生殖细胞发育 germ cell development 

    植物的有性生殖涉及减数分裂（孢子发生）和配子体（花粉）发育，受多个基因调控并需要细胞间的通讯和相互协调。我们主要用拟南芥为材料分离突变体，用分子生物学手段研究大小孢子发生及其配子体发育的分子机理。
    sexual reproduction in plants occurs via meiosis (sporogenesis) and gametophyte development under strict genetic control with highly coordination between cells. we use the current powerful tools in cell and molecular biology to study the related molecular mechanisms through isolating mutants in arabidopsis and cloning the corresponding genes.

    先后主持国家自然科学基金面上、重大研究计划和重点项目，以及973项目子课题。目前主持2项国家自然基金在研项目：

    （1）ckrc3在维持拟南芥内源生长素稳态中的作用和机理；

    （2）组蛋白h2b单泛素化修饰对植物内源生长素稳态的影响和作用机理。

    生长素和细胞分裂素是调控植物生长发育的重要激素，对它们作用机理的研究一直是国际上的热点。

    研究成果：（1）是国际上独立发现生长素生物合成ipa途径突变体taa1的4家实验室之一，并进一步独立提出了细胞分裂素通过促进生长素合成并抑制其极性运输的作用机理调控植物发育[plant j 66, 516–527 (2011)]；(2）2004年提出胞间桥/通道功能假说[j theor biol 229: 139-146 (2004)]，在国际上受到关注，被《cell》、《pnas》、《trends mol med》、《biol rev》、《cold spring harb perspect biol》和《dev biol》及相关专著所引用；（3）1990年在国际上公认为难度极大的小麦原生质体培养中首先实现再生细胞直接形成体细胞胚和再生植株 [科学通报(中文和英文版1990)，中国科学（中文版1990，英文版1991)]。

现每年可同时招收硕士及博士研究生。

部分发表论文（按年份顺序排列）

• 郭光沁、夏光敏、李忠谊、陈惠民，1990，小麦原生质体培养体细胞胚直接发生再生植株。科学通报 35(6): 480.
• guo gq, xia gm, li zy, chen hm, 1990, direct somatic embryogenesis and plant regeneration in protoplast culture of wheat.  chin sci b (sci bull) 35(17): 1495-6.
• 郭光沁、夏光敏、李忠谊、陈惠民，1990，小麦原生质体再生细胞直接形成体细胞胚和再生植株。中国科学b辑 20(9): 970-4。
• guo gq, xia gm, li zy, chen hm, 1991, direct somatic embryogenesis and plant regeneration from protoplast-derived cells of wheat. sci in china (ser b) 34(4): 438-45.
• 郭光沁、许智宏、卫志明、陈惠民，1993，用peg法向小麦原生质体导入外源基因获得转基因植株。科学通报 38(13): 1227-31。
• guo gq, xu zh, wei zm, chen hm, 1993, trangenic plants obtained from wheat protoplasts transformed by peg-mediated direct gene transfer. chin sci b (sci bull) 38(24): 2072-8.
• guo gq, maiwald f, lorenzen p & steinbiss hh, 1998, factors influencing t-dna transfer into wheat and barley cells by agrobacterium tumefaciens. cereal res comm 26: 15-22.
• 李卫、郭光沁*、郑国锠，2000，根癌农杆菌介导遗传转化研究的若干新进展。科学通报 45(8): 798-807。
• li w, guo gq*, zheng gc, 2000, agrobacterium-mediated transformation: state of the art and future prospect. chin sci b (sci bull) 45(17): 1537-46.
• guo gq*, zheng gc, 2004, hypotheses for the functions of intercellular bridges in male germ cell development and its cellular mechanisms. j theor biol 229: 139-146.
• wang qf, li w, yang hai, liu yl, cao hh, dornmayr-pfaffenhuemer m, stan-lotter h, guo gq*, 2007, halococcus qingdaonensis sp. nov., a halophilic archaeon isolated from a crude sea-salt sample. int j syst evol microbiol 57: 600-604.
• liu y, yan z, chen n, di x, huang j, guo g*. 2010. development and function of central cell in angiosperm female gametophyte. genesis (dev genet) 48: 466-478.
• zhou zy, zhang cg, wu l, zhang cg, chai j, wang m, jha a, jia pf, cui sj, yang m, chen r, guo gq*. 2011. functional characterization of the ckrc1/taa1 gene and dissection of hormonal actions in the arabidopsis root. plant j 66: 516–527.
• wang l, sun c, wang zh, guo gq*（联系人）. 2012. mechanism of apoptotosis induced by ortho-topolin riboside in human hepatoma cell line smmc-7721. food chem toxico 50, 1962-1968.
• wu l, pan l, di dw, wang l, wang m, lu ck, wei sd, zhang l, zhang tz, amakorová p, mrvková m, novák o, guo gq*. 2015. forward genetic screen for auxin-deficient mutants by cytokinin. sci rep 5, 11923; doi: 10.1038/srep11923.
• wu l, zhou zy, zhang cg, chai j, zhou q, wang l, hirnerová e, mrvková m, novák o, guo gq*. 2015. functional roles of three cutin biosynthetic acyltransferases in cytokinin responses and skotomorphogenesis. plos one 10(3): e0121943; doi: 10.1371/journal.pone.0121943.
• di dw, zhang c, luo p, an cw, guo gq. 2015. the biosynthesis of auxin: how many paths truly lead to iaa? plant growth regulation 78, 275-285.
• di dw, zhang c, guo gq. 2015. involvement of secondary messengers and small organic molecules in auxin perception and signaling. plant cell rep 34(6):895-904.
• zhao l, liu p, guo g*, wang l*. 2015. combination of cytokinin and auxin induces apoptosis, cell cycle progression arrest and blockage of the akt pathway in hela cells. mol med rep 12: 719-727.
• di dw, wu l, zhang l, an cw, zhang tz, luo p, gao hh, kriechbaumer v, guo gq*. 2016. functional roles of arabidopsis ckrc2/yucca8 gene and the involvement of pif4 in the regulation of auxin biosynthesis by cytokinin. sci rep 6, 36866; doi: 10.1038/srep36866.
• shah jn, guo gq*, krishnan a*, ramesh m, katari nk, shahbaaz m, abdellattif mh, singh sk, dua k. 2021. peptides-based therapeutics: emerging potential therapeutic agents for covid-19 [published online ahead of print, 2021 oct 8]. therapie s0040-5957(21)00204-3. doi:10.1016/j.therap.2021.09.007.
• liu hq, zou yj, li xf, wu l, guo gq*. 2021. stablization of acos by natb mediated n-terminal acetylation is required for ethylene homeostasis. bmc plant biol 21(1):320. doi: 10.1186/s12870-021-03090-7.
• zhang l, luo p, bai j, wu l, di dw, liu hq, li jj, liu yl, khaskheli aj, zhao cm, guo gq*. 2021. function of histone h2b monoubiquitination in transcriptional regulation of auxin biosynthesis in arabidopsis. commun biol 4(1):206. doi: 10.1038/s42003-021-01733-x.
• wu l, wang jl, li xf, guo gq*. 2021. cytokinin-controlled gradient distribution of auxin in arabidopsis root tip. int j mol sci 22(8):3874. doi: 10.3390/ijms22083874. 
• zhou y, zhang j, lin h, guo gq, guo y. 2010. morpheus’ molecule1 is required to prevent aberrant rna transcriptional read-through in arabidopsis. plant physiol 154: 1272–1280.
• zhang cg, guo hh, zhang j, guo gq, schumaker k, guo y. 2010. arabidopsis csaat1a and csaat1b proteins form a complex with cullin4 and ddb1a and regulate the response to uv irradiation. plant cell 22: 2353-2369.